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METHOD OF MULTIPLEX IMMUNOASSAYS UTILIZING DIFFERENTIAL AFFINITY AND METHODS FOR SYNTHESIZING APTAMER-BASED REAGENTS FOR MULTIPLEX IMMUNOASSAYS
METHOD OF MULTIPLEX IMMUNOASSAYS UTILIZING DIFFERENTIAL AFFINITY AND METHODS FOR SYNTHESIZING APTAMER-BASED REAGENTS FOR MULTIPLEX IMMUNOASSAYS
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机译:利用差异亲和力的多重免疫分析方法和合成基于抗原的多重免疫分析试剂的方法
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摘要
Multiplex immunoassays utilize the differential affinities among the conjugation pairs between the capture ligands and target analytes are proposed. Window magnetic-assisted rapid aptamer selection (window-MARAS) methods for selecting aptamers with desirable affinity toward the target analytes and methods for generating reagents for multiplex immunoassays or multiplex detection in one assay by utilizing the selected aptamers as capture ligands in reagents are described and used to demonstrate the feasibility of multiplex immunoassays based on the differential affinity of conjugation pairs between the capture ligands and target analytes.
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