A traditional Chinese medicine composition for promotion of browning of white adipocytes, as well as a preparation method and use thereof are provided. The traditional Chinese medicine composition is made of Radix Astragali seu Hedysari, Piper nigrum and Radix Glycyrrhizae. A natural product for promotion of browning of white adipocytes is prepared by using traditional Chinese medicine components with medicine food homology, which makes up for a blank of products made of natural raw materials in this research field, and can play a role in prevention/treatment of obesity and metabolic diseases associated therewith based on safety.
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