RESONANT STIMULATED RAMAN SCATTERING MICROSCOPE

摘要

A nonlinear optical process on increasing the signal in SRS microscope by Resonant Stimulated Raman Scattering (RSRS) combines both RRS and SRS nonlinear processes, in absorber and host such a β-carotene-methanol solution, Flavins, or other key absorbers in tissues. The observed effect of enhanced RSRS in small signal gain is attributed to RR process in absorber β-carotene that transferring excess vibrations to host methanol from anharmonic vibrational interactions between the solute β-carotene in resonance with the solvent methanol vibrations. SRS microscopy signals are improved in RSRS microscope for imaging vibrational states in lipids CH2 and proteins CH3 in cancer tissues from RR in Flavins or other native chromophores in tissue and for applications in other areas of neuroscience and biomedicine, potentially enhancing signals in the RSRS microscope by 2 to 1000 times.