High speed label-free imaging with molecular specification is highly desired for biomedical and material sciences. Spontaneous Raman microscopy is able to provide vibrational identification of chemical bonds, but often hindered by low sensitivity. Nonlinear stimulated Raman scattering (SRS) microscopy is a novel label-free imaging method with significantly gained signal and image contrast. Moreover, SRS microscopy offers background-free chemical imaging with readily interpretable spectra, and high spatial and temporal resolution. By precise and fast mechanical control, we report high speed SRS monitoring and imaging of chemical reactions in situ. Especially for 3D micro-printing, photoetching and biocompatible hydrogels, high speed and submicron resolution SRS microscopy provides molecular and non-invasive tool for compositional imaging during chemical reaction.
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