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METHOD FOR GENOTYPING MYCOBACTERIUM TUBERCULOSIS
METHOD FOR GENOTYPING MYCOBACTERIUM TUBERCULOSIS
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机译:结核分枝杆菌基因分型的方法
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摘要
The present application provides a method for genotyping M. tuberculosis, comprising obtaining amplifying and obtaining a first DNA fragment from a DNA sample by using one or more primer sets selected from the group consisting of primer sets 1 to 25 (SEQ ID Nos. 1 to 50); amplifying and obtaining a second DNA fragment from the obtained first DNA fragment by using one or more extension primers selected from the group consisting of SEQ ID Nos. 51 to 75; and detecting the second DNA fragment by using mass spectrometry, particularly by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).
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机译:本申请提供了对M进行基因分型的方法。结核病,包括通过使用一个或多个选自引物组1至25(SEQ ID Nos.1至50)的引物组,从DNA样品中扩增并获得第一DNA片段;通过使用一种或多种选自SEQ ID Nos。51至75的延伸引物从获得的第一DNA片段扩增并获得第二DNA片段;通过质谱法,特别是通过基质辅助激光解吸电离飞行时间质谱法(MALDI-TOF MS)检测第二个DNA片段。
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