Remote Focusing All-Optical Digital Scanning Light Sheet Microscopy for Optically Cleared Tissue Sections

摘要

This application discloses a tools and techniques for using a remote-focusing element (such as an electro-tunable lens) to provide enhanced focusing in an all-optical digital scanning microscopy system. The remote-focusing element can imaging of different sections of the specimen without moving the specimen itself, while at the same time providing the ability to correct for non-uniform indices of refraction in the imaged specimen and/or the surrounding medium which can cause misalignment between the imaged plane and the illuminated plane, which can result in loss focus in the imaged section of the specimen. The remote-focusing element can also obviate the need to move the detection stage and/or the illumination stage of the system to image different sections, eliminating vibration during the imaging process.