NOVEL EUKARYOTIC CELL-FREE PROTEIN EXPRESSION SYSTEM THAT DOES NOT REQUIRE AN ARTIFICIAL ENERGY REGENERATION SYSTEM

摘要

This disclosure concerns the systems, methods, and kits for the in vitro synthesis of biological macromolecules in a reaction utilizing cell lysates containing plastids, mitochondria and/or chloroplasts, wherein creatine phosphate and creatine kinase are not added to the reaction to provide artificial energy regeneration.