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Role for the perlman syndrome exonuclease Dis3l2 in the Lin28-let-7 pathway
Role for the perlman syndrome exonuclease Dis3l2 in the Lin28-let-7 pathway
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机译:Perlman综合征核酸外切酶Dis3l2在Lin28-let-7途径中的作用
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摘要
The 3′-5′ exonuclease, Dis3l2, is responsible for the decay of uridylated pre-let-7 miRNA. Biochemical reconstitution assays revealed that 3′ oligouridylation stimulates Dis3l2 activity in vitro, and knockdown of Dis3l2 in mouse embryonic stem cells leads to the stabilization of pre-let-7 miRNA. These Dis3l2-depleted stem cells displayed elevated expression of pluripotency genes and delayed differentiation. The present disclosure establishes 3′ oligouridylation as an RNA decay signal for Dis3l2 and identifies the first physiological RNA substrate of this exonuclease.
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