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Sequence specific primer pool for multiplex PCR and method of detecting microbial infections in thalassemia patients

机译：多重PCR的序列特异性引物库和地中海贫血患者的微生物感染检测方法

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摘要

Reagents and methods for the fast, accurate and early detection of infections in thalassemia major patients. Reagents include a primer pool containing a mixture of primer pairs for the specific recognition and simultaneous amplification of targeted gene sequences of pathogens from a biological sample collected from a patient in a multiplex PCR reaction. Pathogens may include at least one Fungi species, Klebsiella pneumoniae, Candida albicans and Staphylococcus aureus. Subsequent identification of the pathogens is achieved by DNA sequencing analysis.

机译：快速，准确和早期检测地中海贫血严重患者感染的试剂和方法。试剂包括一个引物库，其中包含引物对的混合物，这些引物对用于在多重PCR反应中从患者收集的生物样品中特异性识别并同时扩增病原体的靶向基因序列。病原体可能包括至少一种真菌，肺炎克雷伯菌，白色念珠菌和金黄色葡萄球菌。随后的病原体鉴定是通过DNA测序分析实现的。 展开▼

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公开/公告号US9863009B2

专利类型

公开/公告日2018-01-09

原文格式PDF

申请/专利权人 UNIVERSITY OF DAMMAM;
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申请/专利号US201414518413

发明设计人 AMEIN KADHEM AL-ALI;FRANCIS BORGIO JESU ALEXANDER;ZAKI NASERULLAH;SAYED ABDUL AZEEZ;SANA AL-JARRASH;
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申请日2014-10-20

分类号C12Q1/68;

国家 US

入库时间 2022-08-21 12:54:39

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