A procedure for the extraction and purification of Erythropoietin and / or D - Peptides, peptides and Cu00cdCLICOS peptidomimu00c9tidos used as ligands in this procedure cromatogru00c1ficos

摘要

Describes a procedure for the extraction and / or Erythropoietin purification using Affinity Chromatography Technique. The procedure is particularly useful in the extraction and / or purification of Erythropoietin from supernatant of cell culture or from urine, and includes the use of peptides or peptidomimetics immobilized on a suitable support.The procedure is very efficient due to the High selectivity and Affinity between the Protein and the Peptide, combining the steps of capturing and Fine purification in a single step and the High stability of the peptides against proteases present in the supernatants OF CULT Ivo Cellular.