Biotechnological process and semi continuous production of acid ku00f3jico (PBSC - HMP) using sucrose as a carbon source

摘要

Biotechnological process and semi continuous production of acid ku00f3jico (PBSC - HMP) using sucrose as carbon source.The present invention relates to a process of semi continuous fermentation of sucrose in submerged cultivation of filamentous fungus (Aspergillus flavus Miba - 001)For the production of acid ku00f3jico (HMP: 5 - hydroxy, 2 - hydroxymethyl - range - pirona), (PBSC - HMP)And that is presented as a potential technology in the use of carbon source is abundant and low cost for the generation of a biomolecule that presents several applications of commercial interest; ressaltand- if the volume of implementation, which characterizes this process in a pilot scale, process yield and the production time that characterize this biotechnological process as technically feasible.This process uses means of cultivation of simple composition and there is no pH adjustment.According to oxygen tension of 70% throughout the production process in the second phase of microbial growth (exponential phase).The objective of this invention is to provide a biotechnological process for the production of the HMP from a plentiful source of carbohydrate in advance the addition of inoculation.It should be in the form of pellets formed after 3 days of cultivation, and to provide technical feasibility under suitable conditions for the isolation and purification of the final product.Facilitating the renewal of culture medium and the withdrawal of the product with the extension of the production period in the number of cycles of the process.