Compositions and kits for multiplex genotyping systems via specific molecular microsatellites for piracanjuba (Actinopterygii: Brycon orbignyanus - bryconidae)

摘要

Compositions and kits for multiplex genotyping systems via specific molecular microsatellites for piracanjuba (Brycon orbignyanus – Actinopterygii: bryconidae).The present invention demonstrates implementation processes of systems multiplex molecular characterization microsatellites, via polymerase chain reaction (PCR).The fish piracanjuba (Brycon orbignyanus? Actinopterygii: Characidae), high economic and environmental importance.The processes may also be used for the implementation of compositions and commercial kits to be used, for example,In the food industry and in the management of fish stocks and transactions of stocking.The process makes use of sets of pairs of oligonucleotides primers originally isolated from sequences of regions flanqueadoras microsatellites (short repetitive DNA sequences)The piracanjuba and, associated with specific sets, have the ability to reveal more quickly and economically.Genetic profiles and patterns of genetic variability in the target species of the invention, piracanjuba, a fish spawning with great potential is still largely unexplored for aquaculture.