The use of dimetilsulfoniopropionato (DMSP) as a precursor of metanotiol for production of L - methionine by using a heterologous microbial metabolic route.

摘要

The present invention describes the use of dimetilsulfoniopropionato (DMSP) for the generation of metanotiol precursor used as donor of methyl enxore and radical for the synthesis of L - methionine.We describe the generation of a biosynthetic pathway characterized by heterologous enzymes from marine organisms incorporated in eschericchia coli and / or Corynebacterium glutamicum for su00ednteIf l - methionine in the fermentation process.This invention presents in detail the genetic modifications necessary for the mentioned bacteria are able to consume, and convert the DMSP on various substrates.In a sequence of enzymatic reactions, until the formation of L - methionine. The bioprocess in question is feasible through the use of industrial waste containing glycerin, together or not with glucose.The present invention aims to use a source of reduced sulphur as an alternative to currently used and represents a viable bioprocess and low environmental impact.