selection of extracellular matrix components and / or matrix proteins for improved cell viability and retention after cryopreservation

摘要

The present invention relates to a method for cryopreservation of substrate-bound adherent cells and a method for identifying one or more extracellular matrix (ecm) components and / or matrix proteins that improve the viability and retention of cryopreserved cells in the substrate. . The method for cryopreservation includes treating a substrate with at least one ecm component and / or adding at least one matrix protein to the cell substrate or culture medium, coating the cells on the treated substrate and cryopreserving the cells. One or more components of the ECM and / or matrix proteins that improve cell viability and retention may be identified by evaluating cells that have been thawed from cryopreservation temperature to determine cell viability and retention.