Method for the characterization of variable region gene of receptor of T cells or immunoglobulin rearranged

摘要

Methods of analysis of a region of a nucleic acid marker, detection or monitoring of a clonal population of cells in a mammal.Diagnostic and / or monitoring a condition of disease of mammal and enrichment of a population of nucleic acid molecules in a biological sample.The present invention relates to a method for characterization of a region of nucleic acid and, more particularly, to a method of analysis of a region of the nucleic acid marker.The method of the present invention is based on the identification of one or both of the regions flanking a region of nucleic acid nucleic acid marker and provides a means of analysis of a reg.Ion of nucleic acid marker that is characteristic of a clonal population of cells.The method of the present invention is useful in the context of allow for a variety of applications, including, but not limited to,The monitoring of the progression of a condition characterized by the presence of a clonal population of cells (such as neoplastic conditions).Monitoring of the levels of one or more clonal populations of cells, prediction of the possibility of recurrence of an individual in a state of content to a state of sick,To assess the effectiveness of existing therapeutic drug and / or new therapeutic agents, and identification of the presence of a marker region.