首页> 外国专利> Method for identifying the plant or plasma germinating of Cucumis melo and composition comprising a pair of amplification primers

Method for identifying the plant or plasma germinating of Cucumis melo and composition comprising a pair of amplification primers

机译:鉴定黄瓜黄瓜的植物或血浆发芽的方法以及包含一对扩增引物的组合物

摘要

Methods for conveying Fusarium oxysporum f.sp. melonis (FOM) race 1,2 resistance into non-resistant melon germplasm are provided, in some embodiments, the methods include introgressing FOM race 1,2 resistance into a non-resistant melon using one or more nucleic acid markers for marker-assisted selection among melon lines to be used in a melon breeding program, wherein the markers are linked to FOM race 1,2 resistance. Also provided are quantitative trait loci (QTLs) associated with resistance to FOM race 1,2; isolated and purified genetic markers associated with FOM race 1,2 resistance; melon plants, seeds, and tissue cultures produced by any of the disclosed methods; fruit and seed produced by the disclosed melon plants; and compositions including amplification primer pairs capable of initiating DNA polymerization by a DNA polymerase on melon nucleic acid templates to generate melon marker amplicons.
机译:运送枯萎镰刀菌的方法提供了向非抗性瓜种质中的瓜类(FOM)1,2族抗性,在一些实施方案中,所述方法包括使用一种或多种核酸标记物将FOM 1,2族抗性渗入非抗性瓜子以进行标记辅助选择在瓜育种程序中使用的瓜系中,其中的标记与FOM第1,2种抗性相关。还提供了与FOM第1,2种抗性相关的数量性状基因座(QTL);与FOM种族1,2抗性相关的分离和纯化遗传标记;通过任何公开的方法产生的瓜类植物,种子和组织培养物;由公开的瓜类植物产生的水果和种子;组合物和包括扩增引物对的组合物,所述扩增引物对能够通过DNA聚合酶在瓜子核酸模板上引发DNA聚合以产生瓜果标记扩增子。

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