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Means and methods for vitamin b12 production in duckweed

机译:浮萍中维生素B12的生产方法和方法

摘要

The present invention is directed to a system and method for producing vitamin B12 enriched Duckweed Bacterial Culture (DBC) composition. The aforementioned method comprises steps of inoculating at least one Lemnoideae species and at least one vitamin B12 producing bacteria species in a volume of growth media; incubating said at least one Lemnoideae species and said at least one vitamin B12 producing bacteria species under predetermined conditions to provide a Duckweed-Bacterial Culture (DBC); determining time intervals within said plots characterized by DBC with highest vitamin B12 content; and harvesting said DBC at said predetermined time intervals, thereby providing vitamin B12 enriched DBC composition. The present invention further discloses a composition comprising a vitamin B12 enriched Duckweed-Bacterial Culture (DBC), wherein said composition comprises at least one Lemnoideae species and at least one B12 producing bacteria species, further wherein said vitamin B12 content in said composition is in the range of between about 0.01 and about 100 μg per 100 g of said DBC.
机译:本发明涉及用于生产富含维生素B12的浮萍细菌培养物(DBC)组合物的系统和方法。前述方法包括以下步骤:在一定体积的生长培养基中接种至少一种衣藻科物种和至少一种产生维生素B12的细菌物种;在预定条件下孵育所述至少一种衣藻科物种和所述至少一种产生维生素B12的细菌物种以提供浮萍细菌培养物(DBC);确定在以DBC为特征的最高维生素B12含量的所述地块内的时间间隔;在所述预定时间间隔收获所述DBC,从而提供富含维生素B12的DBC组合物。本发明进一步公开了一种组合物,其包含富含维生素B12的浮萍细菌培养物(DBC),其中所述组合物包含至少一种衣藻科物种和至少一种产生B12的细菌物种,并且其中所述组合物中所述维生素B12的含量在所述组合物中。每100g所述DBC在约0.01至约100μg之间的范围。

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