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Improved bacterial endotoxin test for the determination of endotoxins
Improved bacterial endotoxin test for the determination of endotoxins
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机译:改进的细菌内毒素测试,用于测定内毒素
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摘要
Herein is reported a method for determining bacterial endotoxin at low concentrations in a sample of an antibody (that has been produced using bacterial cells) comprising the following steps in the following order: i) adding magnesium ions to the sample, ii) diluting the sample, iii) dialyzing the sample having a pH-value of 5.7-8.0 against an endotoxin-free aqueous solution, and iv) determining bacterial endotoxin in the sample using a bacterial endotoxin test, particularly the limulus amoebocyte lysate assay.
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