The present invention disclose a robust, efficient and economical process for culturing of four types of cells comprising of Neuronal Cells, Cardiomyocytes, Hepatocytes and Skeletal Muscle cells on a single Tissue Chip Platform without a microfludic method for accelerating drug discovery. The process involves centrifuging whole human blood to separate supernatant layer and the white blood cell layer and transferring the separated supernatant layer in to a flask comprising of RPMI and incubated in 5% CO2 atmosphere to promote adhesion of the Mesenchymal Stem Cells after cell growth to the bottom layer of the flask in which supernatant was discarded and mesenchymal stem cells were mechanically dislodged using cell dislodger and gentle shaking. Then the dislodged mesenchymal stem cells were spinned and seeded into fresh DMEM without glucose and Fetal Bovine Serum and incubated in 5% CO2atmosphere to culture mesenchymal stem cells. The cultured mesenchymal stem cells was next transferred into 4 cell culture flasks with varying proportion of growth factors for differentiation and followed by incubating to form differentiated mesenchymal stem cells. Subsequently transferring the differentiated mesenchymal stem cells in to a tissue chip with Pentagrit Cell Culture Matrix i.e D0422 - Tryptophan, 0.024, Leucine 0.130 (modified composition) followed by incubation to form a 3 dimensional growth of Neuronal Cells, Cardiomyocytes, Hepatocytes and Skeletal Muscle cells on respective matrix. Finally dislodging mechanically the formed Neuronal Cells, Cardiomyocytes, Hepatocytes and Skeletal Muscle cells from the tissue chip thereby the tissue chip serves as a single platform for the growth of four different types of cells at the same time.
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