ELECTRODE, ELECTROCHEMICAL SENSOR AND APPARATUS, AND METHODS FOR OPERATING THE SAME

摘要

pThe application is directed to the amperometric detection of proteins, especially haemoglobin in faeces, using an electrochemical sensor (1). The electrochemical sensor comprises: a working electrode (2a, 2b) having an electrically conductive matrix holding a first reagent and/or a second reagent, the second reagent being an oxidising agent, or a precursor thereof, for the first reagent; a counter electrode (4) and optionally a reference electrode (3); wherein a reaction between the first reagent and the oxidising agent is catalysed by the protein to provide a detectable signal at the working electrode. The electrically conductive matrix is an electrically conductive carbon- or graphite-containing matrix or an electrically conductive porous matrix. The first reagent is preferably 3, 3', 5, 5' - tetramethylbenzidine (CAS RN = 54827-17-7) and the second reagent is hydrogen peroxide, or a precursor such as urea peroxide (CAS RN = 124-43-6), or sodium perborate. Also claimed are a kit for testing stools, comprising the sensor, and a method of preparing the working electrode./p[WO2010055306A1]