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SECRETION OF FUNCTIONAL INSULIN GLARGINE DIRECTLY INTO THE CULTURE MEDIUM THROUGH OVER EXPRESSION OF KEX2P INTRACELLULARLY
SECRETION OF FUNCTIONAL INSULIN GLARGINE DIRECTLY INTO THE CULTURE MEDIUM THROUGH OVER EXPRESSION OF KEX2P INTRACELLULARLY
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机译:通过将KEX2P过表达直接地将功能性胰岛素甘草糖分泌到培养培养基中
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摘要
The disclosure relates to a process of obtaining a fully folded two chain insulin glargine that require no further processing to make it functionally active. The present disclosure discloses a surprising effect of over expression of Kex2p intracellularly under the control of inducible FLD1 promoter in the host Pichia pastoris to produce two chain functional glargine secreted directly in the medium. The schematic digram of how the two chains are made inside the host Pichia pastoris and secretes into the medium. WO2013144685A1
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