SUPERPARAMAGNETIC NANOSTRUCTURED SYSTEM COVERED WITH CHITOSAN FOR IMMOBILIZATION OF MICROORGANISMS AND THE REMOVAL OF LIQUID MEDIUM OR APPLICATION IN FERMENTATION PROCESSES THEREOF.

摘要

The present invention discloses a method for preparing ferrite/metal magnetic nanoparticles, such as cobalt, nickel, copper, among others, coated with chitosan. The said method characterizes by the simultaneous co-precipitation from the mixture of the salts and chitosan upon starting addition of an aqueous solution of one or more precipitation agents to remove the magnetic nanoparticles coated with chitosan by application of the magnetic field. Also, it relates to the use of the superparamagnetic properties of the magnetite/maghemite/chitosan nanoparticles and/or different ferrite nanocomposites coated with chitosan (or another polymer placed on magnetic nanoparticles surface capable of interacting with microbial cells). The said usage needs to interact with bacteria, fungal spores or yeast cells for removal of the liquid medium and/or biofilm formation during subsequent fermentation reuse thereof in the repeated cycles of the fermentation in such a way that the nanostructured system protects the cells against inhibitory effects. In the case of metal-containing nanoparticles with antimicrobial activity, its complex with cells and spores characterizes by the effect of inhibiting the growth of microorganisms isolated by interaction with the nanostructured magnetic system.