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NOVEL BIOLOGICAL DETECTION METHOD FOR DIOXINS IN SERUM, AND A DIAGNOSTIC USE THEREFOR IN METABOLIC SYNDROME AND RELATED CONDITIONS
NOVEL BIOLOGICAL DETECTION METHOD FOR DIOXINS IN SERUM, AND A DIAGNOSTIC USE THEREFOR IN METABOLIC SYNDROME AND RELATED CONDITIONS
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机译:血清中二恶英的新型生物检测方法及其在代谢综合征和相关条件中的诊断用途
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摘要
The present invention relates to a novel biological detection method for dioxins in serum and a diagnostic use thereof in metabolic syndrome and related conditions. More particularly, according to the present invention, a significant correlation between dioxin content in serum and physical variables has been confirmed by measuring luciferase activity through the use of serum as a whole and cells transformed with a recombinant vector comprising a gene construct in which a dioxin responsive element, a promoter and a reporter gene are operably linked. And there is an improvement in this method of the present invention over the conventional methods which require a preprocessing step in order to purify dioxins from serum, by which plural samples can be analyzed easily and accurately even with a very small amount of each because of the use of serum as a whole. Therefore, the detection system of the present invention which involves total serum processing in a genetically transformed cell line comprising a recombinant reporter gene whose expression is modified by dioxin compounds can be effectively used for the biological detection of dioxins in serum. This method can also be used to research the correlation between specific POPs and patient's disease factors by accurate detection of POPs such as dioxins in the serum, and further this method can be effectively used to predict the occurrence of disease and to determine treatability.
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