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METHODS FOR PROVIDING CYTOPLASMIC MALE STERILE PETROSELINUM CRISPUM PLANTS, CYTOPLASMIC MALE STERILE PETROSELINUM CRISPUM PLANTS AND SEEDS AND PLANT PARTS THEREOF
METHODS FOR PROVIDING CYTOPLASMIC MALE STERILE PETROSELINUM CRISPUM PLANTS, CYTOPLASMIC MALE STERILE PETROSELINUM CRISPUM PLANTS AND SEEDS AND PLANT PARTS THEREOF
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机译:提供细胞质雄性不育油橄榄植物的方法,细胞质雄性不育油橄榄植物及其种子和植物的部位
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摘要
The present invention relates to methods for providing cytoplasmic male sterile Petroselinum plants, cytoplasmic male sterile Petroselinum erispum plants and to seeds, cells, tissues and plant parts thereof. Specifically the present invention relates to a method for providing a cytoplasmic male sterile Petroselinum erispum plant comprising: (a) providing first protoplasts obtained from a plant selected from the group consisting of Daucus carota L., Foeniculum vulgare Mill., Apium graveolens L., and Pastinaca saliva L., wherein the first protoplasts have a substantially inactivated nuclear genome and a substantially non-modified cytoplasm; (b) providing second protoplasts obtained from Petroselinum erispum wherein the second protoplasts have a substantially inactivated cytoplasm and a substantially non-modified nuclear genome; (c) fusing said first and second protoplasts; and (d) obtaining from the fusion product of said first and second protoplasts a cytoplasmic male sterile Petroselinum erispum plant..
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