prepared autologous mononuclear cells from peripheral blood were by hypoxia

摘要

The object of the present invention is an autologous preparation (A) substantially free of red blood cells and neutrophil-depleted, comprising peripheral blood mononuclear cells (PBMNCs), Dimethylsulfoxide (DMSO) in a concentration comprised between 0.3% and 1.3% (v/v), autologous platelet-enriched plasma (B) or platelet concentrate (B'). A further object of the present invention is the method for producing the autologous preparation (A), obtained by diluting in said autologous platelet-enriched plasma (B) or in platelet concentrate (B'), an autologous concentrate (C), comprising said peripheral blood mononuclear cells and Dimethylsulfoxide in a concentration comprised between 3% and 5%.