Fluorescent fusion polypeptide, said polypeptide comprising biosensor and uses thereof

摘要

Polypeptide fluorescent melting capable of changing its location within the cell from the cell cytoplasmic membrane to the vesicle retention after an increase in the concentration of second messengers within the cell cytoplasm, comprising a peptide of membrane localization, a peptide binding protein transduction second messengers, a retention signal reticle and a fluorescent peptide in which: a. peptide membrane localization is located in the N-terminus of the polypeptide fluorescent melting end and is physically linked, optionally through a linker, the fluorescent peptide, which in turn is physically connected, optionally through a linker , the peptide binding protein transduction second messengers; and b. peptide binding protein transduction second messenger is physically linked, optionally through a linker, to the latch signal in the reticulum, which in turn is located at the C-terminus of the polypeptide fluorescent melting; and wherein the term "peptide membrane localization" is intended to mean a peptide whose natural intracellular localization is in the plasma membrane.