Simultaneous and integrated selection and evolution of performance and expression of human proteins in production hosts

摘要

A method of evolution of a human template protein in a production host that is a eukaryotic cell production host; Understanding the method: a. evolve the human template protein to produce a set of mutant human proteins in a eukaryotic cell production host; b. examine the set of human mutant proteins for at least one predetermined property, characteristic or activity; C. selecting a superior mutant human protein from the set of mutant human proteins based on the at least one predetermined property, characteristic or activity; and d. manufacturing the superior mutant human protein comprising expressing the superior mutant human protein in the same eukaryotic cell production host as used in step (a) of making it evolve.