Production of mono- and dipegilated IL-10 and its uses

摘要

A method of producing a mixture of mono- and dipegylated IL-10, in which at least one PEG molecule covalently binds at least one amino acid residue of at least one IL-10 subunit, comprising: a) making react from 1 mg / ml-12 mg / ml of IL-10 protein with an activated PEG-connector, so that the ratio of IL-10 to PEG-connector is 1: 1 to 1: 7.7, in presence of 25 mM or 35 mM of a reducing agent, at a pH of 5.0 to 7.4 and a temperature of 5 ° C to 30 ° C, for 3-24 hours, b) purify the IL-mono mixture - and dipegilated.