Production of coagulation factor IX with improved glycosylation pattern

摘要

A method of producing coagulation factor IX with an improved glycosylation pattern in a cell culture, comprising: culturing mammalian cells containing a gene encoding a coagulation factor IX in a cell culture medium comprising manganese between 10 nM and 600 nM under conditions and for sufficient time to allow the expression of coagulation factor IX, in which the glycosylation pattern of the expressed coagulation factor IX is broader than the glycosylation pattern observed under otherwise identical conditions. in an otherwise identical medium lacking manganese, in which a broader glycosylation pattern is a greater number of sugar residues covalently linked in the oligosaccharide chain.