Method of generating L-lysine by means of fermenting bacteria that possess an aconite gene and / or a modified regulatory element

摘要

A method of producing L-lysine by fermentation or to increase the fermentation performance of Llisin, comprising the steps of: (1) modifying an aconitase gene and / or a regulating element thereof on a chromosome of a bacterium producing L-lysine, so that the activity and / or the amount of expression of the bacterial aconitase is reduced, but not eliminated; and (2) produce L-lysine by fermentation with the bacteria obtained by modifying step (1), in which the modification of the aconitase gene and / or a regulatory element thereof includes: (i) a codon substitution start of the nucleotide sequence of SEQ ID NO: 1 of the aconitase gene on the chromosome of a L-lysine producing Escherichia coli by GTG; (ii) a substitution of the nucleotide sequence of SEQ ID NO: 4 of a promoter of the aconitase gene in the chromosome of an L-lysine producing Escherichia coli by the nucleotide sequence of SEQ ID NO: 3; (iii) a substitution of the nucleotide sequence of SEQ ID NO: 6 of a promoter of the aconitase gene in the chromosome of an L-lysine-producing Corynebacterium glutamicum or Corynebacterium pekinense by the nucleotide sequence of SEQ ID NO: 5; (iv) a 90 nucleotide deletion before the stop codon in the nucleotide sequence of the aconitase gene of SEQ ID NO: 1 on the chromosome of an L-lysine producing Escherichia coli; or (v) an addition to the nucleotide sequence of a transcription repressor (SEQ ID NO: 7) of the aconitase gene, preferably an addition of the nucleotide sequence shown in SEQ ID NO: 8 and 7 in tandem.