Screening test to detect the presence of oncogenic HPV viruses

摘要

Method to determine if a sample is positive for oncogenic human papillomavirus (HPV), the method comprising: combining the sample with primers for real-time polymerase chain reaction (PCR) amplification of a target region of genomes of Oncogenic HPV, and a pan-HPV-labeled probe capable of emitting a signal after amplification of the target region, providing conditions for real-time PCR, and measuring a signal emitted from the pan-HPV-labeled probe, where detection of the signal indicates that the sample is positive for oncogenic HPV, characterized in that the pan-HPV probe is a labeled blocked nucleic acid (LNA) molecule comprising the sequence ACTTTCGTTT (SEQ ID No. 25) or its reverse complement.