首页> 外国专利> METHOD FOR DETECTING, QUANTIFYING AND/OR IDENTIFYING AEROMONAS SALMONICIDASPP. SALMONICIDA

METHOD FOR DETECTING, QUANTIFYING AND/OR IDENTIFYING AEROMONAS SALMONICIDASPP. SALMONICIDA

机译：检测，量化和/或鉴定气单胞菌 SPP的方法。沙门氏菌

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摘要

The present invention relates to a method for detecting, quantifying and/or identifying Aeromonas salmonicida spp. salmonicida in a real-time PCR assay. The method comprises: performing a real-time PCR assay on samples of pure or mixed bacterial cultures, on DNA isolated from pure or mixed bacterial cultures or on fish tissues, using a pair of primers specific for Aeromonas salmonicida spp. salmonicida; and detecting, quantifying and identifying the amplified nucleotide sequences. The invention also relates to a kit for detecting, quantifying and identifying Aeromonas salmonicida spp.salmonicida, which comprises said primers, fragments of control DNA, deoxyribonucleotide triphosphates, a reaction buffer and an instruction manual. 展开▼

机译：本发明涉及一种用于检测，定量和/或鉴定鲑鱼气单胞菌的方法。实时荧光定量PCR检测鲑鱼科。该方法包括：使用一对鲑鱼气单胞菌特异的引物，对纯或混合细菌培养物的样品，分离自纯或混合细菌培养物的DNA或鱼组织进行实时PCR分析。鲑科;检测，定量和鉴定扩增的核苷酸序列。本发明还涉及一种用于检测，定量和鉴定鲑鱼气单胞菌的试剂盒，所述试剂盒包括所述引物，对照DNA的片段，脱氧核糖核苷酸三磷酸，反应缓冲液和说明手册。展开▼

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公开/公告号WO2017194808A1

专利类型

公开/公告日2017-11-16

原文格式PDF

申请/专利权人 UNIVERSIDADE DE SANTIAGO DE COMPOSTELA;
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申请/专利号WO2017ES70293

发明设计人 FERNÁNDEZ ÁLVAREZ CLARA;FERNÁNDEZ GONZÁLEZ SANTIAGO;SANTOS RODRÍGUEZ YSABEL;
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申请日2017-05-10

分类号C12Q1/68;

国家 WO

入库时间 2022-08-21 12:47:12

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1. 一种利用RNA恒温扩增的沙门氏菌(Sal.spp.)核酸检测试剂盒 [P] . 中国专利： CN103305593B . 2016.09.28

2. 一种利用RNA恒温扩增的沙门氏菌(Sal.spp.)核酸检测试剂盒 [P] . 中国专利： CN103305593A . 2013-09-18

3. Process for the detection, quantitation and / or identification of Aeromonas salmonicida spp. salmonicida [P] . ES2578367B2 . 2016-12-01

机译：用于检测，定量和/或鉴定鲑鱼气单胞菌的方法。鲑科

4. Procedure for the detection, quantification and/or identification of aeromonas salmonicida spp. Salmonicide (Machine-translation by Google Translate, not legally binding) [P] . ES2578367A1 . 2016-07-26

机译：沙门氏菌气单胞菌的检测，定量和/或鉴定程序。杀人药（由Google翻译进行机器翻译，不具有法律约束力）

5. PROBE FOR AEROMONAS SALMONICIDA AND THE USE THEREOF IN METHODS OF DETECTING OR DETERMINING THE ORGANISM [P] . 欧洲知识产权局专利： EP0618982B1 . 1996-03-13

机译：鲑气单胞菌的探针及其在检测或确定有机体的方法中的用途

1. Isolation and Characterization of a New Subspecies of Aeromonas salmonicida (Aeromonas salmonicida subsp.Flounderacida subsp.Nov.) from Stone Flounder(Kareius bicoloratus L.) [J] . Zhang Xiaojun(张晓君), Zhan Wenbin, Chen Cuizhen, 高技术通讯（英文版） . 2004,第004期

2. Implications of Electrical Impedance-Based Microbiological Technology in Pork Meat Processing Industry for the Rapid Detection and Quantification of Salmonella Spp. [J] . Cristina, Pablos, Javier, 食品科学与工程：英文版 . 2017,第001期

3. Effective qPCR methodology to quantify the expression of virulence genes in Aeromonas salmonicida subsp salmonicida [J] . Rivera L., Lopez-Patino M. A., Milton D. L., Journal of applied microbiology . 2015,第4期

4. Ulcerative dermatitis caused by Aeromonas salmonicida spp. salmonicida in farmed Atlantic salmon in British Columbia. [J] . J R Brocklebank The Canadian veterinary journal . 1998,第2期

5. AsaGEI2b: a new variant of a genomic island identified in the Aeromonas salmonicida subsp salmonicida JF3224 strain isolated from a wild fish in Switzerland [J] . Emond-Rheault Jean-Guillaume, Vincent Antony T., Trudel Melanie V., FEMS Microbiology Letters . 2015,第13期

6. A wavelet-based method to detect, locate, quantify and identify slight deviations of amplitude and phase in power systems [C] . Chen Xiangxun . 2003

7. Detection of fish pathogens with PCR-DGGE in non-lethal mucus samples and molecular typing of Aeromonas salmonicida using the 16S-23S internal transcribed spacers. [D] . Quinn, Robert Andrew. 2008

8. Ulcerative dermatitis caused by Aeromonas salmonicida spp. salmonicida in farmed Atlantic salmon in British Columbia. [O] . J R Brocklebank 1998

9. Effective qPCR methodology to quantify the expression of virulence genes in Aeromonas salmonicida subsp. salmonicida [O] . L. Rivera, M.A. López-Patiño, D.L. Milton, 2015

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2. 鱼类致病杀鲑诺卡氏菌(Nocardia salmonicida)特异性PCR检测方法的建立 [J] . 夏洪丽 ,鲁义善 ,汤菊芬 . 广东海洋大学学报 . 2014,第004期

3. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004）（P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期

4. 浦东地区某种鸽场沙门氏菌的分离鉴定及耐药性检测 [C] . 俞向前 ,韩少鹏 ,刘耀芳 . 2012年华东地区农学会学术年会 . 2012

5. 肠炎沙门氏菌的分离鉴定与特异性检测方法的建立 [A] . 陆广富 . 2010