LENTIVIRAL VECTOR FOR SPECIFICALLY KNOCKING DOWN HUMAN MIRNA-29A AND MIR-140 EXPRESSIONS, AND APPLICATION THEREOF

摘要

A lentiviral vector for specifically knocking down human miRNA-29a and miR-140 expressions, and application thereof. The lentiviral vector comprises a fundamental sequence, a resistance gene sequence, a multiple cloning site sequence, a promoter sequence, and an oligonucleotide sequence targeting miR-29a and miR-140 of a pLKO.1-puro expression vector. A multiple cloning site comprises an Age I enzyme cutting site and an EcoR I enzyme cutting site; the oligonucleotide sequence targeting miR-29a and miR-140 is inserted into the multiple cloning site in sense orientation. A pLKO-Tud-29a-140 lentiviral expression vector has the advantages of high transfection efficiency, a small amount required, and continuous, efficient, stable and specific inhibition in human miRNA-29a-140 expression, and may serve as a powerful tool for preparing drugs for treating diseases related to abnormal miRNA-29a-140 expression.