Provided are an isolated promoter SAFES6 not expressed in endosperm and an application of the promoter SAFES6. Specifically, provided are a promoter SAFES6 capable of transcribing a heterologous nucleic acid sequence in plant tissue rather than in endosperm, and methods for preparing and using the promoter. Also provided are an expression cassette containing the promoter, a recombinant expression vector, and a method for obtaining a corresponding transgenic plant.
展开▼