DNA-BINDING PROTEIN USING PPR MOTIF AND USE OF SAID DNA-BINDING PROTEIN

摘要

The present invention addresses the problem of generalizing and improving a DNA-binding protein in which a PPR is used. Provided is a protein including at least one PPR motif having a structure represented by formula (1), wherein one PPR motif (M_n) included in the protein is a PPR motif having three amino acids of No. 1 A.A., No. 4 A.A., and No. "ii" (-2) A.A., in a combination of specific amino acids corresponding to a target DNA base or a target DNA base sequence, and satisfies at least one selected from the group consisting of the following (a)-(h): (a) No. 7 A.A. in the PPR motif (M_n) is isoleucine (I); (b) No. 9 A.A. in the PPR motif (M_n) is alanine (A); (c) No. 10 A.A. in the PPR motif (M_n) is tyrosine (Y); (d) No. 18 A.A. in the PPR motif (M_n) is lysine (K), arginine (R), or histidine (H); (e) No. 20 A.A. in the PPR motif (M_n) is glutamic acid (E) or aspartic acid (D); (f) No. 29 A.A. in the PPR motif (M_n) is glutamic acid (E) or aspartic acid (D); (g) No. 31 A.A. in the PPR motif (M_n) is isoleucine (I); and No. 32 A.A. in the PPR motif (M_n) is lysine (K), arginine (R), or histidine (H).