METHOD FOR BUILDING LIBRARY AND METHOD FOR SNP TYPING

摘要

Provided is a method for building a library, which comprises: A. performing PCR amplification on a sample to be sequenced containing a SNP site to be detected using a specific amplification primer set to obtain an amplification product; at least one primer in the primer set contains an IIS type restriction enzyme recognition sequence, and the amplification product contains an IIS type restriction enzyme cleavage site, and the distance between the IIS type restriction enzyme cleavage site and the SNP site to be detected is 0 to 5 bases; B. using the IIS type restriction enzyme to cleave the amplification product to obtain a first nucleic acid fragment containing the SNP site to be detected, and forming a first end by enzyme cleaving the first nucleic acid fragment; and C. ligating the first nucleic acid fragment with a sequencing linker at the first end under a ligase to obtain a library molecule. Also provided is a method for SNP typing. The method shortens the detection time for sites, improves the accuracy of the detection, and unifies the sequencing primers for detecting multiple sites in the same system.