METHOD FOR BUILDING LIBRARY AND METHOD FOR SNP TYPING

摘要

Provided is a method for building a library, which comprises: PCR amplifying a sample to be sequenced to obtain an amplification product; ligating linker one onto the amplification product to obtain a ligation product containing an IIS type restriction enzyme recognition sequence and an IIS type restriction enzyme cleavage site, and linker one is a double-stranded nucleic acid molecule, and the distance between the IIS type restriction enzyme cleavage site and the SNP site to be detected is 0 to 5 bases; using the IIS type restriction enzyme to cleave the ligation product to obtain a first nucleic acid fragment containing the SNP site to be detected and linker one, and forming a first end by enzyme cleaving the first nucleic acid fragment; ligating linker two at the first end of the first nucleic acid fragment to obtain a library molecule, and linker two is a double-stranded nucleic acid molecule containing a binding site of the sequencing primers. Also provided are a SNP typing method and a kit for detecting a SNP site mutation. The method shortens the detection time for sites, improves the accuracy of the detection, and unifies the sequencing primers for detecting multiple sites in the same system.