STEPPED MERGED INJECTION FOR SURFACE PLASMON RESONANCE ASSAYS

摘要

A device (60) and method for producing data for correction curves for SPR assays provides a plurality of different concentrations of solvent or other compound to the reference surface and active surface of a SPR chip. The concentrations are mixed online by two pumps (79, 77), one connected to a source (73) of a first concentration of solvent or other compound and the other connected to a source (71) of a second, different concentration of solvent or compound. Mixing of the two different concentrations of solvent or compound can take place in a fluid mixer (75) and the concentration of solvent or compound in the mixture leaving the fluid mixer (75) can be varied by adjusting the relative speeds of the pumps (79, 77). The concentration of the solvent or compound mixture can be adjusted during injection of the mixed liquids in order to provide the different concentrations necessary to make a solvent correction curve.