CRISPR/CAS9 HIGH EFFICIENT GENE EDITING SYSTEM OPTIMIZED FOR KLUYVEROMYCES

摘要

A CRISPR/Cas9 safe and high efficient gene editing system for Kluyveromyces comprises Cas9/gRNA fusion plasmids, wherein the plasmids target the endogenous DNA sequence of Kluyveromyces and produce a double strand break, and further comprises a donor DNA sequence for transforming into Kluyveromyces cells, wherein the sequence generates homologous recombination with the target site at the double strand break to insert a Tag sequence into the target site. The gene editing system can stably replicate, express, and perform gene modification in Kluyveromyces.