METHOD FOR GENERATING A MULTIPLE-GENE ASSEMBLY IN A SINGLE DNA CONSTRUCT

摘要

Described is a method for generating a multiple-gene assembly in a single DNA construct, a kit and a single DNA construct generated by the method. Different assembly methods are known in the state of the art but user has to decide between scar free, sequence independent methods, which require the time consuming individual design of every assembly and modular approaches, which generate scar sequences and require laborious parts domestication. In order to overcome these drawbacks a method for generating a multiple- gene assembly in a single DNA construct is provided that contains freely designed scar-free level 0 assembly units in a predefined order, determined by flanking homology regions and which is sequence-independent.