CONTROL OF MICROBIAL NANOCELLULOSE FILM PROPERTIES AND PRODUCTION VOLUME BY TUNING PHYSICAL CULTURE CONDITIONS

摘要

The properties of microbial pellicles are tuned by adjusting the physical culture conditions. A culture of Gluconacetobacter xylinus can be grown in a liquid growth media having a surface exposed to air so that a basal pellicle of microbial cellulose forms on the surface. Feeding the culture by adding additional liquid growth media at the surface, thereby submerging the basal pellicle; and then allowing the culture to grow again forms a second pellicle of microbial cellulose.