PERIPHERAL-BLOOD CIRCULATING TUMOR CELL DETECTION SYSTEM AND APPLICATION THEREOF

摘要

Provided is a peripheral-blood circulating tumor cell detection system. In particular, provided is a detection system containing a buffer system and a V2C conjugated magnetic bead; in addition, a V2C protein variant is also provided; the preferred buffer system contains: glycerin, 4-hydroxyethylpiperazine ethanesulfonic acid (HEPES), and a phosphate buffer solution; the pH of said buffer system is 7.0-7.6, and a pH of 7.2-7.4 is preferable; the V2C conjugated magnetic bead is a complex obtained by conjugating a V2C protein or variant thereof with a magnetic bead. Also provided are a test kit and reaction system containing said buffer system. The buffer system and V2C conjugated magnetic bead are used for CTC detection, and has excellent performance in comparison with existing CTC detection means.