MODULATION OF ENZYMATIC POLYNUCLEOTIDE SYNTHESIS USING CHELATED DIVALENT CATIONS

摘要

Methods and apparatus of modulating polynucleotide synthesis are provided. The methods include delivering reagents comprising enzymes, nucleotides and ions to oligonucleotide primers wherein the reagents catalyze incorporation of the nucleotides to 3' ends of the oligonucleotide primers, and modulating incorporation of the nucleotides to the 3' ends of the oligonucleotide primers. Polynucleotide synthesis is modulated by modulating presence or absence of catalytic cation cofactors to provide sequence defined synthesis of polynucleotides. In certain embodiments, the polynucleotides encode information.