Methods for the synthesis of plinucleotidos FROM ENZYMATIC oligonucleotide Assembly,For the preparation of a Library of polynucleotides and for identification of polynucleotides from a Library of polinucleotridos.

摘要

Preparation Methods of arbitrary large polynucleotide sequences and in a manner that will easily to their Automation.The Method comprising polynucleotides having at least 200 nucleotides in either direction 5 to 3 or 3 to 5 by the connection of a Plurality of Oligonucleotides.The Assembly which represents the desired Nucleotide sequence of the Gene.Libraries of polynucleotides are also provided for Members of polynucleotides having desired Properties.