METHOD AND KIT FOR PREPARING ANTIBODY PAIR AND USE OF KIT, AND SYSTEM FOR PREPARING ANTIBODY PAIR

摘要

Disclosed are a method and a kit for preparing an antibody pair and the use of the kit, and a system for preparing the antibody pair. The method comprises: using an antigen-binding fragment of an existing antibody as a capture antibody and using an anti-crystallizable fragment antibody as a labelled antibody, and directly screening a target antibody that can be paired with the existing antibody from the supernatant of a cell culture. The method can be used to directly screen the supernatant of the cell culture for obtaining the paired antibody, and does not require a large number of processes in terms of preparation, purification and labelling, etc., for the antibodies to be screened, thereby greatly reducing the workload.