METHOD FOR CONSTRUCTING CIRCULAR RNA HIGH-THROUGHPUT SEQUENCING LIBRARY AND KIT THEREOF

摘要

Disclosed are a method for constructing a circular RNA high-throughput sequencing library and a kit thereof. The construction method sequentially comprises the steps of: (S1) extracting total RNA from a sample; (S2) removing DNA from the sample; (S3) detecting and evaluating the quality of the total RNA in the sample, and determining that the RNA quality meets requirements; (S4) removing rRNA; (S5) removing linear RNA; and (S6) constructing the circular RNA library for high-throughput sequencing. The method for constructing the circular RNA high-throughput sequencing library is high in efficiency and stable, and has a low residual ratio of the rRNA, is high in circular RNA detection efficiency, good in data repeatability, and has a high verification success rate for the sequencing results, and is especially applicable to an FFPE tissue and other samples with a poor RNA quality.