METHODS AND SYSTEMS FOR ADAPTING PATHLENGTH AND/OR WAVELENGTH OF A UV-ABSORBANCE CELL IN A CHROMATOGRAPHY SYSTEM

摘要

The present invention relates to a method for determining operational status of a chromatography column (1; 39, 47, 59; 107, 109, 111, 113), comprising detecting a feed signal (21; 201) representative of the composition of a feed material provided to the inlet of the column; detecting the UV absorbance in the feed material, detecting an effluent signal (23; 203, 205, 207, 209) representative of the composition of the effluent from the column; and using the feed signal and the effluent signal to determine operational status of the column. The feed signal is generated using a first UV detector having a first UV cell pathlength operating at a first UV wavelength and the effluent signal is generated using a second UV detector having a second UV cell pathlength operating at a second UV wavelength. The method further comprising determining a first threshold value based on the detected UV absorbance in the feed material, and selecting the first UV cell pathlength and/or first UV wavelength based on the first threshold value.