首页>
外国专利>
A CELL-BASED ASSAY AND KITS FOR ASSESSING SERUM ANTICHOLINERGIC ACTIVITY
A CELL-BASED ASSAY AND KITS FOR ASSESSING SERUM ANTICHOLINERGIC ACTIVITY
展开▼
机译:基于细胞的血清抗线虫活性评估试剂盒
展开▼
页面导航
摘要
著录项
相似文献
摘要
Provided herein are methods for determining the level of muscarinic acetylcholine receptor subtype- (M1 receptor) anticholinergic activity in a blood serum sample, the method comprising the steps of removing protein from the blood serum sample by treatment with perchloric acid (PCA) to produce a PCA-treated serum sample; incubating the PCA-treated serum sample with a membrane preparation from cultured cells expressing the M1 receptor and an M1 receptor ligand; detecting an amount of binding of the M1 receptor ligand to the M1 receptor and comparing the amount of binding to a standard to determine the level of M1 receptor anticholinergic activity in the blood serum sample. Also provided are kits for performing the method.
展开▼