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>An Experiment on Standardized Cell Culture Assay in Assessing the Activities of Composite Artemisia Capillaris Tablets against Hepatitis B Virus Replication in vitro
An Experiment on Standardized Cell Culture Assay in Assessing the Activities of Composite Artemisia Capillaris Tablets against Hepatitis B Virus Replication in vitro
Objective: To explore the activities of Composite Artemisia Capillaris Tablet (复方茵陈片, CACT) against hepatitis B virus replication in vitro . Methods: By means of radioimmunoassay (RIA), Dot blot and Southern blot, the surface and e antigen production of 2.2.15 cells, HBV DNA in 2.2.15 cell culture medium and that in 2.2.15 cells were examined respectively. Results: HBsAg, HBeAg values of 2.2.15 cells treated by CACT were lower than those of the control, the HBV DNA quantities in culture medium and in 2.2.15 cells decreased as compared with those cells with no treatment by CACT given to them. Conclusion: CACT could inhibit HBV DNA replication, showing its potential antiviral activity in hepatitis B treatment.
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机译:The interaction of hepatitis a virus (HaV) with soluble forms of its cellular receptor 1 (HaVCR1) share the physiological requirements of infectivity in cell culture