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ACCURATE, RAPID AND CONVENIENT SINGLE-STEP DISEASE DIAGNOSTIC METHOD USING SELF-AMPLIFICATION PRINCIPLE OF DETECTION SIGNAL
ACCURATE, RAPID AND CONVENIENT SINGLE-STEP DISEASE DIAGNOSTIC METHOD USING SELF-AMPLIFICATION PRINCIPLE OF DETECTION SIGNAL
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机译:使用检测信号自放大原理的准确,快速,便捷的单步病诊断方法
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摘要
The present invention relates to a method and a kit for accurately, rapidly, and conveniently detecting a disease marker by linking immunodiagnostics and Au particle formation at the same time and realizing self-amplification of a detection signal and, more particularly, to a method and a kit for detecting a disease-specific marker by using self-amplification of a detection signal, the method comprising the steps of: (a) simultaneously inducing an antigen-antibody immune response and an Au particle formation reaction by reduction of Au ions in an assay solution produced by adding a sample and a reducing agent into a pre-assay solution, wherein the pre-assay solution comprises simultaneously antibodies or antigens for detection of a disease-specific marker, free Au ions and adsorbing Au ions, and the sample contains disease-specific antigens or antibodies specifically binding to the antibodies or the antigens; and (b) confirming the presence or absence of a disease-specific marker by a chromogenic reaction through the Au particle formation. The method according to the present invention can diagnose a disease in a single step without any processes such as immobilization and washing processes that usually accompany a conventional diagnosis such as ELISA; can be effectively used for diagnosis of diseases, in particular in patients in an emergency, since the time required for a diagnostic test, which conventionally took several hours or even several days, is as short as about 10 minutes; and furthermore, is useful because a detection result can be confirmed by the naked eye without a separate analyzer, and therefore, the detection cost can be minimized.
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